DNA sequencing has provided a new approach for studying evolutionary relationships, since: 1. All organisms have a genome 2. The genes that code for vital cellular functions are conserved to a remarkable degree through evolutionary time 3. Even these genes accumulate random changes with time (usually in the regions that are not vital for function). In this respect the gene [...]
In this article we will discuss about Recombinant DNA Technology:- 1.Steps in Recombinant DNA Technology 2. Tools for Recombinant DNA Technology 3. Techniques Used In Recombinant DNA Technology 4. Applications of Recombinant DNA Technology. Steps in Recombinant DNA Technology: Basic steps involved in rec DNA technology (or genetic engineering) are given below (Fig. 1): i. Selection and isolation of DNA [...]
The following points highlight the seven steps involved in the preparation of a recombinant DNA. The steps are: 1. Selection of Target DNA 2. Selection of a Suitable Cloning Vector DNA or Vehicle DNA 3. Selection of Restriction Endonucleases 4. Procedure for Production of Recombinant DNA (rDNA) 5. Introduction of the rDNA into a Host Cell 6. Selection of the [...]