The following points highlight the top five techniques of chromosome banding. The techniques are: 1. C-Bands 2. G.Bands 3. Q-Bands 4. N-Bands 5. Other Techniques of Chromosome Banding. Chromosome Banding: Technique # 1. C-Bands: The technique of C-banding originated after the work of Pardue and Gall who reported that constitutive heterochromatin can be stained specifically by Giemsa-solution. Each chromosome possesses [...]
In this article we will discuss about the heterochromatin and euchromatin in eukaryotic chromosomes. The terms "heterochromatin" and "euchromatin" were given by Heitz in 1928-29, although they had been discovered much earlier. Heterochromatic blocks observed during interphase were earlier termed as pro-chromosomes. The substance of which eukaryotic chromosomes are composed is known as chromatin; it contains DNA, protein and a [...]
In this article we will discuss about the changes in chromatin structure during transcription. Displacement of nucleosomes: During transcription, some changes in the chromatin structure occur in the region of transcription. RNA polymerase is comparatively larger in size than the nucleosomes. The molecular weight of RNA polymerase is about 5,00,000 Daltons, while that of nucleosome is about 2,60,000 Daltons. Lewin [...]