As medium engineering means designing (micro) environments for bio-cells/biocatalyst with the objective of reaching maximal activity, stability and hence productivity it exerts, therefore, great influence on bio-reaction kinetics. Production of gluconic acid as discussed above provides a good example of the same. The scheme of this bio-reaction is as below. As found experimentally one mole of glucose gives one mole [...]
In bioorganic reactions a biocatalyst in a liquid medium is surrounded by various micro environments. The biocatalyst may be an enzyme, or more complex systems such as microorganisms, eukaryotic cells or parts thereof. The micro-environments consist of a thin layer of water around the biocatalyst and an interfacial region. This forms the transition between the bio-catalytic and continuous phases. Medium [...]
In any process biotechnology consideration, material balance is essential for the sake of metabolic economy. Frameworks using material balances in metabolic pathways to study cellular metabolism has been investigated by various researchers. From material balances the concept of stoichiometric coefficients for the overall reaction representing the biological system has been introduced and was determined on line to provide information about [...]