In this article we will discuss about the Cell Suspension Culture:- 1. Meaning of Cell Suspension Culture 2. Initiation of Cell Suspension Culture 3. Medium 4. Aeration 5. Significance. Meaning of Cell Suspension Culture: Cell suspension culture is the culture of cells or cell aggregates dispersed in a moving liquid medium. Batcher and Ingram ('76) noted that all suspension cultures [...]
The below mentioned article provides a study notes on Single Cell Clone. By culturing a single isolated cell single cell clone can be obtained. Single cell clones derived from callus or suspension culture are heterogenous. They differ from one another in shape, texture, friability, colour, growth and morphogenetic potential etc. Working with Nicotiana tabacum L. Winsconsin ('38), Murashige and Nakamo [...]
The below mentioned article provides a quick notes on Isolation of Protoplasts and Protoplast Culture. Klerker (1892) made unsuccessful attempts to isolate protoplasts. In 1909 Kunster isolated protoplasts by mechanical method. Effective technique of protoplast isolation was evolved by Cocking ('60), Nagata and Takebe ('70) and Bhojwani and Cocking ('72). Enzymes used for isolation of protoplasts are cellulase, hemicellulase and [...]