The following points highlight the two main types of suspension culture. The types are: 1. Batch Culture 2. Continuous Culture. Type # 1. Batch Culture: A batch culture is a cell suspension culture grown in a fixed volume of nutrient culture medium, cell suspension increases in biomass by cell division and cell growth until a factor in the cultural environment [...]
In this article we will discuss about single cell culture in plants, explained with the help of suitable diagrams. The single cells can be isolated from a variety of tissue and organ of intact plant or from callus tissue or from cell suspension. Single cells can be isolated from intact plant tissue or callus tissue by using either mechanical method [...]
In this article we will discuss about:- 1. Sources of Explant for Protoplast Isolation 2. Methods of Protoplast Isolation 3. Purification of Protoplasts 4. Isolation of Sub-Protoplasts 5. Protoplast Viability Test. Each plant cell has a definite cellulose wall which is attached with each other by middle lamella made up of pectin. Protoplast is the content which is surrounded only [...]