The following points highlight the top five utility of suspension culture. The utilities are: 1. Source of Secondary Metabolites 2. Biotransformation 3. Source of Somatic Embryos and Cell Line 4. Source of Protein 5. Mutant Production and its Use. Utility # 1. Source of Secondary Metabolites: Cell suspension cultures have been shown to produce a number of valuable seconÂdary metabolites, [...]
In this article we will discuss about single cell culture in plants, explained with the help of suitable diagrams. The single cells can be isolated from a variety of tissue and organ of intact plant or from callus tissue or from cell suspension. Single cells can be isolated from intact plant tissue or callus tissue by using either mechanical method [...]
In this article we will discuss about:- 1. Sources of Explant for Protoplast Isolation 2. Methods of Protoplast Isolation 3. Purification of Protoplasts 4. Isolation of Sub-Protoplasts 5. Protoplast Viability Test. Each plant cell has a definite cellulose wall which is attached with each other by middle lamella made up of pectin. Protoplast is the content which is surrounded only [...]