In this article we will discuss about the initiation and maintenance of callus culture. Callus culture represents clumps of unorganised parenchyma tissue formed through vigorous proliferation by cell division from any kind of explants under cultural condition, showing no polarity. Any kind of permanent but living tissue such as parenchyma, collenchyma, cortical tissue, pith cells, phloem tissue, cambium cells or [...]
In this article we will discuss about single cell culture in plants, explained with the help of suitable diagrams. The single cells can be isolated from a variety of tissue and organ of intact plant or from callus tissue or from cell suspension. Single cells can be isolated from intact plant tissue or callus tissue by using either mechanical method [...]
In this article we will discuss about:- 1. Sources of Explant for Protoplast Isolation 2. Methods of Protoplast Isolation 3. Purification of Protoplasts 4. Isolation of Sub-Protoplasts 5. Protoplast Viability Test. Each plant cell has a definite cellulose wall which is attached with each other by middle lamella made up of pectin. Protoplast is the content which is surrounded only [...]