In this article we will discuss about the initiation and maintenance of callus culture. Callus culture represents clumps of unorganised parenchyma tissue formed through vigorous proliferation by cell division from any kind of explants under cultural condition, showing no polarity. Any kind of permanent but living tissue such as parenchyma, collenchyma, cortical tissue, pith cells, phloem tissue, cambium cells or [...]
The below mentioned article provides a study note on suspension culture. A cell suspension culture consists of cell aggregates dispersed and growing in moving liquid media. It is normally initiated by transferring pieces of undifferentiated and friable calli to a liquid medium agitated by a suitable device (Fig. 17.3A-C). Agitation in the medium helps in two ways, it exerts a [...]
The following points highlight the two main types of suspension culture. The types are: 1. Batch Culture 2. Continuous Culture. Type # 1. Batch Culture: A batch culture is a cell suspension culture grown in a fixed volume of nutrient culture medium, cell suspension increases in biomass by cell division and cell growth until a factor in the cultural environment [...]