Read this article to learn about the types, uses, design and precautionary measures of centrifuges. Introduction: A centrifuge is a device for separating particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed. In a solution, particles whose density is higher than that of the solvent sink (sediment), and particles that are lighter [...]
Read this article to learn about the basic principles of sedimentation and also about sedimentation coefficient. Basic Principles of Sedimentation: The rate of sedimentation is dependent upon the applied centrifugal field (G) being directed readily outwards; this is determined by the square of the angular velocity of the rotor (ω in radians s-1) and the radians (r, in centimeters) of [...]
This article throws light upon the three methods used for separation of particles in centrifugation. The three methods are: (1) Differential Centrifugation (2) Centrifugal Elutriation and (3) Density Gradient Centrifugation. The Density Gradient Centrifugation method consists of two techniques. The two techniques are: (1) Rate Zonal Technique and (2) Isopycnic Centrifugation Technique. Method # 1. Differential Centrifugation: This depends upon [...]