In this article we will discuss about the relations among nucleic acids, nucleotides and nucleosides. Nucleic Acids: These are defined as polynucleotides composed of repeating units ribonucleotides (in RNA) or deoxyribonucleotides (in DNA). The difference between the two structure is that in deoxyribonudeotides there is no-OH group in position 2'. The presence of three esterifiable-OH groups in the ribose portion [...]
In this article we will discuss about the Extraction and Estimation of Deoxyribonucleic Acid (DNA). Principle: Extraction of DNA is accomplished by the rupturing of cell wall and nuclear membrane followed by deproteinization and precipitation of the nucleic acid using ethanol. Materials: a. Sample Material b. Extraction Medium 0-15 M NaCl, 0-1 M Na2 c. Lysozyme solution, 10 mg/ml d. [...]
In this article we will discuss about the Extraction and Estimation of Ribonucleic Acid (RNA). Principle: The ribonucleoprotein complex is dissociated by SDS into RNA and protein, deproteinized by phenol and the free RNA left in aqueous solution is precipitated in the cold alter adding alcohol. Materials: a. Centrifuge (bench top) b Magnetic stirrer c. Cold room (4°C) d. Phenol [...]