The below mentioned article provides an overview on Ribonucleic Acid (RNA). In broad features, the chemistry of RNA is comparable to the chemistry of DNA. The chemical analysis shows the following two main differences in the polynucleotide chains of DNA and RNA: (i) The sugar in RNA is ribose whereas it is deoxyribose in DNA. (ii) While in DNA, the [...]
In this article we will discuss about the Extraction and Estimation of Ribonucleic Acid (RNA). Principle: The ribonucleoprotein complex is dissociated by SDS into RNA and protein, deproteinized by phenol and the free RNA left in aqueous solution is precipitated in the cold alter adding alcohol. Materials: a. Centrifuge (bench top) b Magnetic stirrer c. Cold room (4°C) d. Phenol [...]
The following points highlight the three types of ribonucleic acid (RNA). The three types are: 1. Messenger RNA (mRNA) 2. Transfer RNA (tRNA) and 3. Ribosomal RNA (rRNA). RNA Type # 1. Messenger RNA (mRNA): It carries the genetic message from the chromosomes (DNA) to the ribosome (site of protein synthesis). This RNA is formed in the nucleus (or nuclear [...]