The following points highlight the four main features that are required to facilitate cloning into a vector. The features are: 1. Origin of Replication 2. Selectable Marker 3. Cloning (Recognition) Sites 4. Vectors for Cloning Genes in Plants and Animals. Feature # 1. Origin of Replication (Ori): This is a sequence from where replication starts and any piece of DNA, [...]
In this article we will discuss about the Tools of Systematic Botany:- 1. Geographic Information System 2. Molecular Techniques. 1. Geographic Information System (GIS): The latest technique used as a tool in Biosystematics is Remote sensing and the use of computer. Remote sensing is carried out by satellites to get an idea of the particular phytogeographical area. It was first [...]
In this article we will discuss about the Centrifugation Technique of Molecular Biology:- 1. Basic Principle of Centrifugation Technique 2. Types of Centrifugation Technique. Basic Principle of Centrifugation Technique: The principle of the centrifugation technique is to separate the particles suspended in liquid media under the influence of a centrifugal field. These are placed either in tubes or bottles in [...]