Methods for Obtaining Single Cell Clones from Callus Culture | Plant Tissue Culture

The below mentioned article provides a study notes on Single Cell Clone. By culturing a single isolated cell single cell clone can be obtained. Single cell clones derived from callus or suspension culture are heterogenous. They differ from one another in shape, texture, friability, colour, growth and morphogenetic potential etc. Working with Nicotiana tabacum L. Winsconsin ('38), Murashige and Nakamo [...]