In this article we will discuss about the the structure of larger and smaller subunits of ribosomes. Ribosomes are ribonucleoprotein particles present in all types of cells. They were first observed in EM by Claude in the cytoplasm of cells, and later on the surface of endoplasmic reticulum by Porter and Palade. Ribosomes occur in 3 sizes: 70S in bacteria [...]
The reconstitution of ribosomes has been a dramatic advance of recent times. When ribosomes are centrifuged in a gradient of 5M CsCl, about 30 to 40% of the protein is lost. Both the subunits dissociated into 2 inactive core particles which contain the RNA and some proteins (core proteins). Simultaneously some other proteins called split proteins (SP) are released from [...]
In his article we will discuss about the role of nucleolus in production of ribosomes (explained with diagram). The nucleolus is the site for rRNA transcription and processing as well as for assembly of ribosomal subunits. The nucleolus is organised at the nucleolus organising regions (NORs) of some chromosomes. These chromosomal regions contain the genes for 5.8S, 18S and 28S [...]