Extraction and Estimation of Ribonucleic Acid (RNA)
In this article we will discuss about the Extraction and Estimation of Ribonucleic Acid (RNA). Principle: The ribonucleoprotein complex is dissociated by SDS into RNA and protein, deproteinized by phenol and the free RNA left in aqueous solution is precipitated in the cold alter adding alcohol. Materials: a. Centrifuge (bench top) b Magnetic stirrer c. Cold room (4°C) d. Phenol [...]