Although substantial progress was made during the last decade, all replication mechanisms in eucaryotes are not yet understood because: (a) Replication is an event which occurs much more rarely than in bacteria. Initiation of replication is therefore highly controlled; this complicates the understanding of this process; (b) The DNA is not naked, in the cell, but in the form of [...]
The replication of numerous procaryotes was studied in recent years. The mechanisms of the replication of phages T4, T7 and λ are now well known, while those of B. subtilis are beginning to be understood. However, the most extensively studied system remains Escherichia coli, thanks to the combination of various types of approaches. Experimental Approaches to the Study of Replication: [...]
In this article we will discuss about the fidelity of the replication of DNA. The DNA being the carrier of genetic information it is essential that this information remains correct. But the DNA can be damaged by the action of chemical or physical agents. The cell therefore possesses repair systems which can maintain the integrity of genetic information. It is [...]