The following points highlight the eight important steps in maturation of proteins. The steps are: 1. Protein folding and Chaperones 2. Enzymes in Protein Folding 3. Protein Cleavage 4. Glycosylation 5. Modification by Lipids 6. Protein Degradation 7. The Ubiquitin-Proteasome Pathway 8. Lysosome Mediated Proteolysis. Step # 1. Protein Folding and Chaperones: The experiments of Anfinsen and collaborators half a [...]
In this article we will discuss about the role of proteins in pollen grains. T. Singer and Petrovskya - Baranova (1961) were the first to show the existence of proteins with enzymatic properties in certain layers of the pollen walls of Paeonia and Amaryllis. Subsequently, Makinen and McDonald (1968) demonstrated the pollen enzymes and isoenzymes in flowering plants. The details [...]
The below mentioned article includes a list of three experiments on proteins. 1. Experiment to localize proteins in a cell: Requirements: Fresh tissues, razor, acid fuchcin, acetic acid, glycerine jelly, slides, cover slips. Method: 1. Cut the thin sections of fresh tissues with the help of razor, put them on slide and incubate them in 0.005% acid fuchcin in 1% [...]