In this article we will discuss about the various structures of proteins. This will also help you to draw the structure and diagram of proteins. Proteins consist of long polypeptide chains and we know how to determine the sequence of the amino acids of these chains. This constitutes the primary structure of proteins. But in reality, each protein has a [...]
In this article we will discuss about the structure of immunoglobulins, explained with the help of a suitable diagram. Identification of antibodies in the serum protein fraction was demonstrated in the classic experiments of A. Tiselius and E.A. Kabat in 1939. However, the basic structure of —globulin molecule was revealed by Rodney R. Porter and Gerald Edehman in 1950s and [...]
The important techniques involved in the study of molecular biology of proteins are:- 1. Purification from Cell Extracts 2. Using Column Chromatography 3. Affinity Chromatography 4. Separation of Proteins on Polyacrylamide Gels 5. Antibodies can be Visualized Electrophoretically: Separated Proteins 6. Sequencing Protein Molecules. Technique # 1. Purification from Cell Extracts: Purification of proteins is a major part of understanding [...]