In this article we will discuss about:- 1. Meaning of PCR 2. Steps of PCR 3. Applications of PCR. Meaning of PCR: In genetic engineering, requirement of desirable amount of target DNA is indispensable to study genetic structure, function and gene manipulation. It is novel cell free techniques to obtain innumerable number of copies. This direct enzymatic amplification process is [...]
The below mentioned article provides a note on Polymerase Chain Reaction (PCR). Gene Amplification: Polymerase Chain Reaction (PCR): PCR provides a simple and ingenious method for exponential amplification of specific DNA sequences by in vitro DNA synthesis, i.e., this technique has made it possible to synthesize large quantities of DNA fragments without cloning it. Kary Mulis in 1985 developed the [...]
In this article we will discuss about:- 1. Introduction to PCR 2. Technique of PCR 3. Modified Forms. Introduction to PCR: PCR provides a simple and ingenious method for exponential amplification of specific DNA sequences by in vitro DNA synthesis, i.e., this technique has made it possible to synthesize large quantities of DNA fragments without cloning it. Kary Mulis in [...]