Everything you need to know about plasmids. Some of the frequently asked questions are as follows:- Q.1. What is a Plasmid ? Ans. The plasmids have been reported from prokaryotic as well as eukaryotic cells. A plasmid may be defined as extra-chromosomal genetic element which is not essential for the growth of the cell and has no extra cellular form. [...]
In this article we will discuss about:- 1. Meaning of Plasmids 2. Preparation of Plasmids 3. Major Groups. Meaning of Plasmids: Plasmids are dispensable, extra pieces of DNA found mostly in bacteria, that exist separately from the main bacterial chromosome. They are simpler in organisation than viruses, have no protein coat, and cannot exist outside a cell. They replicate independently [...]
In this article we will discuss:- 1. Definition of Plasmids 2. Physical Nature and Copy Number of Plasmids 3. Properties 4. Incompatibility 5. Types 6. Replication 7. Plasmid Curing 8. Use of Plasmids as Coning Vectors. Definition of Plasmids: In addition to bacterial chromosome (nucleoid), bacterial cells normally contain genetic elements in their cytoplasm. These genetic elements exist and replicate [...]