The following points highlight the five main steps involved in purification of plant viruses. 1. Infected leaves are thoroughly homogenized in water or preferably in phosphate, borate or citrate buffer in an electric grinder or in a mortar with pestle (Fig. 13.15). 2. Tissue homogenate is strained through a piece of muslin cloth (or cheese cloth). Crude sap which comes [...]
In this article we will discuss about the structure and replication of Tobacco Mosaic Virus (TMV). Structure of Tobacco Mosaic Virus (TMV): TMV is a simple rod-shaped helical virus (Fig. 13.20) consisting of centrally located single- stranded RNA (5.6%) enveloped by a protein coat (94.4%). The rod is considered to be 3,000 Å in length and about 180 Å in [...]
The below mentioned article provides quick notes on plant viruses. Plant viruses are a persistent threat to production of vegetable crops in India. Although severe outbreaks of viral diseases tend to occur on a sporadic basis, many plant viruses are ubiquitous in nature and can result in severe outbreaks under the right conditions. Virus diseases result in losses through reduction [...]