The following points highlight the six important methods of tissue culture. The methods are: 1. Hanging Drop Culture 2. Double Cover Slip Method 3. Culture in Stewards Auxophyton 4. Culture in Bottles, Conical Flasks etc. 5. Culture in a Micro-Chamber 6. Culture in Petridish, Watch Glass etc.. Method # 1. Hanging Drop Culture: The simplest method of tissue culture is [...]
In this article we will discuss about the Root Culture:- 1. Medium and Methods of Root Culture 2. Legumes of Root Culture 3. Nature 4. Significance. Medium and Methods of Root Culture: Kotte and Robbins ('22) first successfully cultured wheat root tips for a short period. In 1934 White first successfully cultured tomato roots for an unlimited period in a [...]
In this article we will discuss about the Culture of Shoot Apex and Leaf. Culture of Shoot Apex: White ('33) first attempted to culture the shoot apex of Stellaria media, but he was unsuccessful. Loo ('45) successfully cultured 5 mm long excised shoot tips of Asparagus officinalis. Cultured shoots produce cladophylls, which on subculture produce plantlets. On agar medium containing [...]