In this article we will discuss about the basic laboratory requirements for plant tissue culture. Laboratory Requirements: 'Plant tissue culture' or in vitro cultivation of plant parts needs some basic requirements: (a) Cultivation should be done under aseptic conditions. (b) The isolated plant part should get an appropriate environment which will help to divide the cell and to get an [...]
The below mentioned article provides a study note on organogenesis. In plant tissue culture, organogenesis is a process of differentiation by which plant organs like roots, shoots, buds etc. are formed from the unusual points of origin of an organized explants where a preformed meristem is lacking. Plant development through organogenesis is the formation of organs either de novo or [...]
List of top two experiments on plant tissue culture:- 1. Isolation of Explants, Establishment and Maintenance of Callus 2. Culture of Anthers and the Establishment of Haploid Plants. Experiment # 1. Isolation of Explants, Establishment and Maintenance of Callus: Explants isolated from the tissues of higher plants are brought into culture. It requires a nutrient medium consisting of a mineral [...]