In this article we will discuss about the mechanism of antisense ribosome. Persistent search for novel vehicle for introducing antisense RNA into cells has become reality when Sweeney and Chaoyao (1998) developed antisense ribosomes in Tetrahymena thermophila. This system allows expression of antisense gene present with rRNA genes and antisense RNA alters expression function as a part of a stable [...]
Molecular markers are DNA sequences, whose inheritance pattern can be established. Classic examples of molecular markers are: 1. Restriction Fragment Length Polymorphism (RFLP) 2. Randomly Amplified Polymorphic DNA (RAPD) 3. Amplified Fragments Length Polymorphism (AFLP) 4. Sequence Characterized Amplified Region (SCAR). Example # 1. Restriction Fragment Length Polymorphism (RFLP): This novel non-PCR based approach is based on hybridization of probe [...]
In this article we will discuss about the mode of action of antisense RNA. Several possible mechanisms are involved during antisense inhibition of specific gene. Following are some of the most promising mode of actions have been suggested (Fig. 22.3). Action in the Nucleus Premise: One of the earliest methods in antisense inhibition is carried out by blocking transcription. This [...]