The following points highlight the two main types of antisense methods that can be employed to inhibit the expression of a target gene. These antisense methods are antisense oligonucleotides and antisense RNA. All these methods are basically designed to blockade or degradation of a specific target mRNA as a consequence of binding of nucleic acids complementary to sequence of the [...]
Molecular Markers in Breeding Programme: The advent of molecular techniques played a significant role in increase our knowledge of cereal genetics and behaviour of cereal genomics. While RFLP markers have been the basis for most work in crop plants, valuable markers have been generated from RAPD and AFLPs. Recently, other improvised molecular marker such as simple sequence repeats (SSR), microsatellite [...]
In this article we will discuss about the mechanism of antisense ribosome. Persistent search for novel vehicle for introducing antisense RNA into cells has become reality when Sweeney and Chaoyao (1998) developed antisense ribosomes in Tetrahymena thermophila. This system allows expression of antisense gene present with rRNA genes and antisense RNA alters expression function as a part of a stable [...]