Amino acids will be incorporated into proteins from the aminoacyl-tRNAs. To study this process we must acquire a wider knowledge of ribosomes and the messenger RNA, which will form a complex with the aminoacyl-tRNAs. Ribosomes: These are ribonucleoprotein particles consisting of about 65% RNAs and 35% proteins in the case of E.coli and which may be obtained from cellular homogenates [...]
In this article we will discuss about the formation of purine and pyrimidine deoxyribonucleosies. Formation of Purine Deoxyribonucleosides-5'- Triphosphates: The deoxyribonucleotides are simply formed by reduction of the corresponding ribonucleotides. Thus reduction of carbon 2' of ribose, takes place in E.coli and animal tissues at the ribonucleosides-5'-diphosphates stage, at the cost of a protein of low molecular weight, thioredoxin, comprising [...]
The biosynthesis of the DNA must consist in a replication or duplication. The study of the DNA structure as proposed by Crick and Watson (see fig. 6-15) gives an idea of the mechanism of this replication. Each nucleotide of the template DNA chain need only be recognized by a complementary nucleotide before the latter is incorporated at the end of [...]