Some important antibiotics which inhibit the protein synthesis in prokaryotes along with the reason are given below:- 1. Streptomycin: Because it binds to the S12 protein of the 30 S ribosomal subunit and therefore, inhibits binding of tRNA to the P site. It also causes misreading in a system that is in the 'act of synthesis. 2. Neomycin, Kanamycin: Both [...]
Everything you need to know about tools and techniques of molecular biology. Some of the most frequently asked questions are as follows:- Q.1. Name a technique used to separate DNA and RNA molecules according to their size. Ans: Gel electrophoresis (Fig. 34.1). Q.2. Why is DNA while subjected to an electric field migrates through the gel towards the positive pole? [...]
The important techniques involved in the study of molecular biology of proteins are:- 1. Purification from Cell Extracts 2. Using Column Chromatography 3. Affinity Chromatography 4. Separation of Proteins on Polyacrylamide Gels 5. Antibodies can be Visualized Electrophoretically: Separated Proteins 6. Sequencing Protein Molecules. Technique # 1. Purification from Cell Extracts: Purification of proteins is a major part of understanding [...]