The following points highlight the three main factors affecting micro-propagation. The factors are: 1. Genotype 2. Media 3. Cultural Conditions. Factor # 1. Genotype: Physiological conditions of the donor plants influence considerably in the process of morphogenesis. Larger sizes of the meristematic tissues are advantageous. Selecting juvenile parts of the plants yield better results than older ones. The most actively [...]
Plant propagation is carried out by sexual or asexual mode of life cycles. The asexual or vegetative reproduction produces genetically identical plants. Multiplication of several horticulturally important crops follows vegetative methods. Production of genetically identical plant cultivars by asexual method is called clonal propagation and genetically identical individuals derived from a single individual by asexual mode referred as clone. Contrast [...]
According to Murashige of California University, micro-propagation is distinctly divided into five stages. Stage 0 — Mother Plant Selection: Before initiation of micro-propagation, selection of suitable mother plant is crucial in the whole exercise of propagation. Generally, disease free mother explant is selected for the micro-propagation to reduce contamination of cultures. Certain growth parameters of mother plant can be improved [...]