The following points highlight the ten main physical methods of gene transfer. The methods are: 1. Biolystic or Particle Bombardment 2. Electroporation 3. Microinjection 4. Pollen Transformation 5. Liposome Mediated Transfer 6. Microlaser 7. Macro-Injection 8. Silicon Carbide Fiber (SCF) Mediated Transfer 9. Poly Ethylene Glycol (PEG) Mediated Transformation 10. Ultrasound Mediated Transfer. Method # 1. Biolystic or Particle Bombardment: [...]
The following points highlight the top two methods of gene transfer. The methods are: 1. Indirect Method (Agrobacterium Mediated Gene Transfer) 2. Direct Methods of Gene Transfer. Method # 1. Indirect Method (Agrobacterium Mediated Gene Transfer): The transformation of a plant can be carried out directly by using Agrobacterium spp. which is a common bacterium causing crown gall tumour in [...]
In this article we will discuss about the methods of gene transfer. Gene transfer can be done by two methods: (i) Indirect Method through Vectors or Carriers and (ii) Direct or Vectorless Transfer Method. The first method includes: (a) Plasmids and Viruses and (b) Natural Genetic Engineer, (a) Plasmids and Viruses: Recombinant DNA technology involves the transfer of desired genes [...]