The following points highlight the three main types of chromatography. The types are: 1. Adsorption Column Chromatography 2. Thin Layer Chromatography 3. Gas Chromatography. Type # 1. Adsorption Column Chromatography: The general chromatography method was introduced into biology by Tswett in 1906 and various adsorbents such as starch and aluminium oxide are poured as a slurry into a long glass [...]
The following points highlight the top eight spectroscopy techniques. The techniques are: 1. Infrared (IR) Spectrophotometry 2. Circular Dichromism (CD) Spectroscopy 3. Spectrofluorimetry 4. Luminometry 5. Atomic/Flame Spectrophotometry 6. Electron Spin Resonance (ESR) Spectrometry 7. Nuclear Magnetic Resonance (NMR) Spectrometry 8. Mass Spectrometry. Technique # 1. Infrared (IR) Spectrophotometry: IR-light was used in this spectrophotometric analysis, Infra-red spectrophotometry with Gas-Liquid [...]
Although the use of high rotor speeds is not always necessary, most ultracentrifuges are designed to operate safely at rotor speeds of up to 60,000 or even 100,000 rpm. Despite the use of high speed, the sedimenting forces involved are only small and the technique is extremely gentle, providing a valuable method for the separation of large, labile molecules or [...]