Natural occurrence of antisense RNA and its role in gene regulation have been realised long ago. Gene expression in both prokaryotes and eukaryotes are controlled by the product of regulatory gene, which are supposed to be proteins only. Recently, naturally occurring regulatory gene that directs the synthesis of RNA which can control gene expression. This newly discovered RNA repressor can [...]
The following points highlight the three main omic technologies in plant biotechnology. The technologies are: 1. Transcriptomics 2. Metabolomics 3. Phenomics. Technology # 1. Transcriptomics: Utility of microarray and sequence-based process to expression profiling fueled additional approach to current genomic data within bioinformatics. Microarray technology helps in gene expression analysis in many plant species. Sequence based methods have real potential [...]
Realising the role of antisense RNA technology in the reduction of ethylene production, a cDNA (pTOM13) encoding ACC oxidase has been isolated from a tomato fruit ripening library. An antisense gene construct from the cDNA strongly inhibited expression of ACC oxidase activÂity in transgenic tomatoes. A chimaeric pTOM13 antisense gene, encodes ACC oxidase consistÂing of 1.3 kilobase (kb) fragment of [...]