The following points highlight the ten main physical methods of gene transfer. The methods are: 1. Biolystic or Particle Bombardment 2. Electroporation 3. Microinjection 4. Pollen Transformation 5. Liposome Mediated Transfer 6. Microlaser 7. Macro-Injection 8. Silicon Carbide Fiber (SCF) Mediated Transfer 9. Poly Ethylene Glycol (PEG) Mediated Transformation 10. Ultrasound Mediated Transfer. Method # 1. Biolystic or Particle Bombardment: [...]
In this article we will discus about the strategies for stress-tolerant plant. Several microorganisms have been used as a model system to characterize stress inÂduced genes. It is because they are equipped with wide array of stress relieving genes. Recently, attention has been focused towards higher plants in search of stress tolerance genes and sucÂcessful attempt to introduce these genes [...]
In this article we will discuss about the CIS-Acting elements and regulatory role of Dreb-Transcription factors. The promoter region of almost all stress inducible genes contains two major cis-acting elements. One of the most well characterized stress responsive genes, rd 29A (responsive to dehydration) of Arabidopsis contains two kinds of cis-acting elements in their promoter region. One is the ABA-responsive [...]