This article provides a case study on transgenic rice for stress tolerance. Production of agronomically useful transgenic rice has been given top priority in the improvement of crop plants. The loss of rice yield due to stress impact is estimated to cost at least several billion dollars a year. Genetic engineering offers effective way to deal with these problems. Moreover, [...]
Blotting technique is an extremely powerful tool for analyzing gene structure and used to study gene expression, once cloned cDNA is isolated. There are three important types of blotting techniques are: 1. Southern Blotting 2. Northern Blotting 3. Western Blotting. Technique # 1. Southern Blotting: Developed by E.M. Southern, the technique of Southern blotting is one of the most important [...]
The following points highlight the ten main physical methods of gene transfer. The methods are: 1. Biolystic or Particle Bombardment 2. Electroporation 3. Microinjection 4. Pollen Transformation 5. Liposome Mediated Transfer 6. Microlaser 7. Macro-Injection 8. Silicon Carbide Fiber (SCF) Mediated Transfer 9. Poly Ethylene Glycol (PEG) Mediated Transformation 10. Ultrasound Mediated Transfer. Method # 1. Biolystic or Particle Bombardment: [...]