Tag Archives | Genetics

Radioactive and Non-Radioactive Hybridization Methods | Immunology

In this article we will discuss about non-radioactive and radioactive procedures of hybridization. Non-Radioactive Hybridization Procedures: Nucleic acid hybridization can be detected by labeling the probe with a radioactive isotope or a non-radioactive isotope. Therefore, hybridization procedure can be of two types depending upon the type of label used to label the probe. Non-radioactive hybridization procedures have widespread applications in [...]

By |2017-12-12T10:37:14+00:00December 12, 2017|Hybridization Methods|Comments Off on Radioactive and Non-Radioactive Hybridization Methods | Immunology
Read More

Nucleic Acid Hybridization | Tools | Genetics

In this article we will discuss about:- 1. Basic Considerations of Nucleic Acid Hybridization 2. Hybridization Probes 3. Labeling of Hybridization Probes. Basic Considerations of Nucleic Acid Hybridization: Nucleic acid hybridization is a basic tool in molecular genetics which exploits the ability of single stranded nucleic acid molecules to hybridize with complementary sequences to form double stranded molecules. The principle [...]

By |2017-12-12T10:37:14+00:00December 12, 2017|Nucleic Acid Hybridization|Comments Off on Nucleic Acid Hybridization | Tools | Genetics
Read More

Polymerase Chain Reaction-Oligonucleotide Ligation Assay (PCR-OLA) | Genetics

Polymerase Chain Reaction-Oligonucleotide Ligation Assay (PCR-OLA) is a method to diagnose hereditary diseases caused by mutation not affecting restriction endonuclease sites. This method combines Polymer­ase Chain Reaction with the Ligation Assay. PCR-OLA distinguishes between the ligation and the absence of ligation of two oligonucleotides. A single nucleotide mismatch (at the site of hybridized oligonucleotides) prevents ligation and hence we can [...]

By |2017-12-12T10:37:14+00:00December 12, 2017|PCR-OLA|Comments Off on Polymerase Chain Reaction-Oligonucleotide Ligation Assay (PCR-OLA) | Genetics
Read More
Go to Top