In this article we will discuss about the rolling cycle method of DNA replication in φ X 174. All circular genomes do not follow the same pattern of replication described in E. coli. In some bacteriophages (phi, lambda and phi X 174), in mitochondrial chromosomes and during bacterial mating an alternative method known as the rolling circle has been demonstrated. [...]
Some investigators of 1950s focused their attention on viruses containing protein and RNA, but no DNA. The poliomyelitis virus, influenza virus and the virus that causes mosaic disease in tobacco plants (TMV) were thus shown to have genes made of RNA. Earlier in 1935 W. M. Stanley had reported the isolation of a crystalline protein from infected tobacco plants, which [...]
In this article we will discuss about the DNA replication in eukaryotes. In eukaryotes there are only two different types of DNA polymerases in contrast with DNA polymerase I, II and III of prokaryotes. Furthermore the DNA of eukaryotes is a long linear molecule with several replication units. A diploid mammalian cell contains on an average about 6 pg of [...]