The following points highlight the three ways by which sequencing of genes in isolation from rest of human genome takes place. The ways are: 1. Complementary DNA (cDNA) Sequencing 2. Using CpG Island of Locate Genes 3. DNA Sequencing for Wheat Genome. Way # 1. Complementary DNA (cDNA) Sequencing: cDNA is made by converting the mRNA content of cell is [...]
Essay on Gene Therapy! Essay on the History of Gene Therapy: Gene therapy was conceived in 1960, the breakthrough was the synthesis of recombinant DNA molecule (rDNA) in 1972. The rDNA molecules were first duplicated and grown in bacteria in 1973. Later split gene was discovered through the use of recombinant DNA technologies in 1977. Two independent techniques were developed [...]
In this article we will discuss about the regulation of zein gene. The molecular biology of the zein gene system is well established in plant system. Comparison of genomic and cDNA clones have shown that they are totally devoid of intervening sequences. The regulatory signals of zein is TATA box, which is found 30 bp upstream of the transcriptional initiation [...]