Reconstitution of Ribosomes | Genetics
The reconstitution of ribosomes has been a dramatic advance of recent times. When ribosomes are centrifuged in a gradient of 5M CsCl, about 30 to 40% of the protein is lost. Both the subunits dissociated into 2 inactive core particles which contain the RNA and some proteins (core proteins). Simultaneously some other proteins called split proteins (SP) are released from [...]