Tag Archives | Extraction and Estimation

Extraction and Estimation of Deoxyribonucleic Acid (DNA)

In this article we will discuss about the Extraction and Estimation of Deoxyribonucleic Acid (DNA). Principle: Extraction of DNA is accomplished by the rupturing of cell wall and nuclear membrane followed by deproteinization and precipitation of the nucleic acid using ethanol. Materials: a. Sample Material b. Extraction Medium 0-15 M NaCl, 0-1 M Na2 c. Lysozyme solution, 10 mg/ml d. [...]

By |2016-05-16T05:34:24+00:00May 16, 2016|Acids|Comments Off on Extraction and Estimation of Deoxyribonucleic Acid (DNA)
Read More

Extraction and Estimation of Ribonucleic Acid (RNA)

In this article we will discuss about the Extraction and Estimation of Ribonucleic Acid (RNA). Principle: The ribonucleoprotein complex is dissociated by SDS into RNA and protein, deproteinized by phenol and the free RNA left in aqueous solution is precipitated in the cold alter adding alcohol. Materials: a. Centrifuge (bench top) b Magnetic stirrer c. Cold room (4°C) d. Phenol [...]

By |2016-05-16T05:34:24+00:00May 16, 2016|Ribonucleic Acid|Comments Off on Extraction and Estimation of Ribonucleic Acid (RNA)
Read More
Go to Top