In this article we will discuss about the production of various enzymes. 1. Pectinases: During the survey of crops for disease occurrence, it was observed that certain soft-rot causing bacteria produced pectolytic enzymes.The wilt disease induced by Fusarium species also produced pectinases as observed by Gothoskar 1955 and Waggoner and Dimond (1955). It was Scheffer and others who in the [...]
The following points highlight the five main enzymes involved in genetic engineering. The enzymes are: 1. Restriction Endonuclease 2. DNA Ligase 3. Alkaline Phosphatase 4. DNA Polymerase and the Klenow Fragment 5. Reverse Transcriptase. Genetic engineering became possible with the discovery of mainly two types of enzymes: the cutting enzymes called restriction endonucleases and the joining enzymes called ligases. Restriction [...]
The below mentioned article includes a collection of seven experiments on enzyme activity. 1. Experiment to demonstrate the activity of enzymes: Requirements: Benzidine solution, razor, thin sections of actively growing root (or germinating seeds or germinating pollen grains), phosphate buffer, hydrogen peroxide (1%), ammonium chloride (5%), starch paste. Method, observations and results: 1. Cut thin sections of fast-growing roots or [...]