Enzymes Used in Industries and their Uses! Of the 1000 and more enzymes isolated, fewer than 20 are now commercially used on a scale that has significant impact on either the enzyme industry or the user industries. Only the more important will be treated here. It is worth remembering that commercial enzyme preparations represent mixtures in which the principal enzyme [...]
Restriction endonuclease enzymes occur naturally in bacteria as a chemical weapon against the invading viruses. They cut both strands of DNA when certain foreign nucleotides are introduced in the cell. Endonucleases break strands of DNA at internal positions in random manner. Types of Restriction Enzymes: 1. Restriction enzyme Type I: These enzymes interact with an unmodified recognition sequence in double-stranded [...]
In this article we will discuss about the restriction maps and RFLPS. Also learn about the significance of restriction maps. The number of cuts that a restriction enzyme makes in a segment of double-stranded DNA depends on the size of that DNA, its sequence, and the number of base pairs in the recognition sequence of the particular enzyme. That is, [...]