Tag Archives | Enzyme Activity

Estimation of Amylase | Enzyme Activity

In this article we will discuss about the tests for estimation of amylase in plants. Principle: The reducing sugars produced by the action of α- and or β-amylase react with di-nitro-salicylic acid and reduce it to a brown coloured product, nitro-amino-salicylic acid. Materials: i. Sodium acetate buffer, 0.1 M pH 4.7 ii. Starch 1% Solution: Prepare a fresh solution by [...]

By |2016-10-20T07:40:07+00:00October 20, 2016|Plants|Comments Off on Estimation of Amylase | Enzyme Activity
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Factors Affecting the Enzyme Activity | Enzymology

The following points highlight the seven major factors affecting the enzyme activity. The factors are: 1. Temperature 2. Hydrogen Ion Concentration (pH) 3. Water 4. Concentration of the Substrate 5. Enzyme Concentration 6. Inhibitors 7. Accumulation of End-Products. Factor # 1. Temperature: Usually the activity of the enzymes is optimum at normal body temperature at very low temperature (i.e., about [...]

By |2016-09-16T08:12:23+00:00September 16, 2016|Enzymology|Comments Off on Factors Affecting the Enzyme Activity | Enzymology
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