In this article you will discuss about the tests for estimation of catalase in plants. Principle: The enzyme catalase is responsible for catalysing the decomposition of H2O into H2O and O2. The assay of enzyme depends on estimation of residual H2O2 by titration with KMnO4. Requirements: (a) Reagents: 1. H2O2 —0.2 volume 2. H2SO4—10% 3. 0.05 NKMnO4 solution. (b) Glass-wares: [...]
In this article we will discuss about the tests for estimation of cellulase in plants. Principle: Endo-β-1, 4 glucanase acts on carboxymethyl cellulose (CMC) and hydrolyses the β-1, 4 glycosidic bonds in a random manner. As a result, the viscosity of CMC solution is reduced. This is measured in an Ostwald viscometer. Materials: a. Citrate-phosphate Buffer 0.1 M (pH 6.0) [...]
In this article we will discuss about the tests for estimation of ascorbic acid in plants. Principle: Ascorbic acid oxidase acts on the substrate ascorbic acid to form dehydro-ascorbic acid. The residual ascorbic acid content is determined by titration using a dye solution (Dichlorophenol indophenol, an oxidation-reduction dye): Requirements: (a) Reagents: 1. Dye solution: 15 mgm of 2-6 dichlorophenol indophenol [...]