In this article we will discuss about:- 1. Meaning of Stains 2. Purpose of Staining 3. Structural Components 4. Classification. Meaning of Stains: A stain is any colouring organic compound that, when combined with another substance, imparts a colour to that substance. The terms 'dyes' and 'stains' are often used interchangeably by biologists, but they are not the same. The [...]
In this article we will discuss about the staining procedure used for detecting bacteria. 1. Simple Staining Procedure: When a single staining-reagent is used and all cells and their structures stain in the same manner, the procedure is called simple staining procedure. This procedure is of two types - positive and negative (Fig. 17.5). In positive staining, the stain (e.g., [...]
The following points highlight the top three techniques for detection of fungal pathogens. The techniques are: 1. Examination of Seeds without Incubation 2. Incubation Method 3. Seedling Symptoms Test. Technique # 1. Examination of Seeds without Incubation: (i) Dry Seed Examination: Inspection of dry seed can be applied to detect seed- borne pathogen which when present in the seed may [...]