The following points highlight the top three methods of recombinant DNA formation. The methods are: 1. Transformation 2. Transfection 3. Non-Bacterial Transformation. Recombinant DNA Formation: Method # 1. Transformation: The restriction enzyme which causes a break in foreign DNA also causes a staggered cut in the vector DNA at a specific cleavage site. The cohesive ends of vector DNA possess [...]
The following points highlight the two main causes of ploids. The causes are: 1. Aneuploidy 2. Euploidy. Ploids: Cause # 1. Aneuploidy: The individuals carrying chrornosome numbers other than somatic or diploid (2n), which are not true multiples of basic chromosome number or genome are called heteroploids or aneuploids. Thus aneuploidy results due to an addition of loss of less [...]
Subject Matter of Genetic Engineering: DNA is a genetic material which contains all hereditary information needed to create an organism. DNA actually does not make organism, it only makes proteins. DNA is transcribed into mRNA and mRNA is translated into protein and the protein then forms organism. The specific regions on the DNA molecule that direct the synthesis of proteins [...]