In the preparation of culture media, different stock solutions (ATOG) are made. Generally stock solutions of major elements are prepared by 10X final concentration whereas minor elements and vitamins are prepared by 1000X final concentration and stored in the freezer. Stock solutions: A – NH4NO3 B – KNO3 C – CaCl2.2H2O D – KH2PO4 E — Molybdenum CaCl2 KI F [...]
The culture media (nutrients) consist of chemicals which support the growth of culture or microorganisms. Microbes can use the nutrients of culture media as their food is necessary for cultivating them in vitro. Types of Culture Media: The first medium prepared was meat-infusion broth. As most pathogenic microbes require complex food similar in composition to the fluids of the animal [...]
The below mentioned article provides a study note on culture media. Growth and morphogenesis of plant tissues in vitro are largely governed by the composition of the culture media. Although the basic requirements for culturing plant tissue is same but in practice there are some specific nutritional requirements for promoting optimal growth from different kinds of explants in case of [...]