Tag Archives | Cloning Vectors

Features Required to Facilitate Cloning into a Vector

The following points highlight the four main features that are required to facilitate cloning into a vector. The features are: 1. Origin of Replication 2. Selectable Marker 3. Cloning (Recognition) Sites 4. Vectors for Cloning Genes in Plants and Animals. Feature # 1. Origin of Replication (Ori): This is a sequence from where replication starts and any piece of DNA, [...]

By |2016-10-20T07:52:41+00:00October 20, 2016|Recombinant DNA Technology|Comments Off on Features Required to Facilitate Cloning into a Vector
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Vectors Used for Cloning of Larger Molecules of DNA

In this article we will discuss about the three main types of vectors used in cloning for larger molecules of DNA. The types of vectors are: 1. Plasmid Vectors 2. Bacteriophage Vectors 3. Cosmid Vectors. 1. Plasmid Vectors: Plasmids are the extra chromosomal genetic elements commonly found in bacteria and are mostly made of double-stranded circular DNA. They are used [...]

By |2016-08-31T15:41:59+00:00August 31, 2016|Recombinant DNA Technology|Comments Off on Vectors Used for Cloning of Larger Molecules of DNA
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