Tag Archives | cells

Molecular Biology of Proteins: 5 Techniques | Microbiology

The important techniques involved in the study of molecular biology of proteins are:- 1. Purification from Cell Extracts 2. Using Column Chromatography 3. Affinity Chromatography 4. Separation of Proteins on Polyacrylamide Gels 5. Antibodies can be Visualized Electrophoretically: Separated Proteins 6. Sequencing Protein Molecules. Technique # 1. Purification from Cell Extracts: Purification of proteins is a major part of understanding [...]

By |2016-12-20T11:20:17+00:00December 20, 2016|Proteins|Comments Off on Molecular Biology of Proteins: 5 Techniques | Microbiology

Molecular Biology of Nucleic Acids | Microbiology

The techniques used to study molecular biology of nucleic acids are:- 1. Gel electrophoresis 2. Use of restriction endonucleases 3. DNA hybridization 4. Hybridization probes 5. Isolation of specific segments of DNA 6. DNA cloning 7. Cloning DNA in plasmid vectors 8. Vector DNA can be introduced into host organisms by transformation 9. Libraries of DNA molecules can be created [...]

By |2016-12-20T11:20:17+00:00December 20, 2016|Nucleic Acid|Comments Off on Molecular Biology of Nucleic Acids | Microbiology

Characteristics of Biological Lipids | Cells

The chemical, structural and physical characteristics or properties of the biological lipids are as under: (i) The fats and oils are stored forms of energy in living organisms and are highly reduced compounds. (ii) The complete oxidation of fatty acids into CO2 and H20 in cells is highly exergonic or energy producing in the same way as the explosion of [...]

By |2016-12-20T11:20:15+00:00December 20, 2016|Lipids|Comments Off on Characteristics of Biological Lipids | Cells
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