Tag Archives | cells

Molecular Biology of Proteins: 5 Techniques | Microbiology

The important techniques involved in the study of molecular biology of proteins are:- 1. Purification from Cell Extracts 2. Using Column Chromatography 3. Affinity Chromatography 4. Separation of Proteins on Polyacrylamide Gels 5. Antibodies can be Visualized Electrophoretically: Separated Proteins 6. Sequencing Protein Molecules. Technique # 1. Purification from Cell Extracts: Purification of proteins is a major part of understanding [...]

By |2016-12-20T11:20:17+00:00December 20, 2016|Proteins|Comments Off on Molecular Biology of Proteins: 5 Techniques | Microbiology
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Molecular Biology of Nucleic Acids | Microbiology

The techniques used to study molecular biology of nucleic acids are:- 1. Gel electrophoresis 2. Use of restriction endonucleases 3. DNA hybridization 4. Hybridization probes 5. Isolation of specific segments of DNA 6. DNA cloning 7. Cloning DNA in plasmid vectors 8. Vector DNA can be introduced into host organisms by transformation 9. Libraries of DNA molecules can be created [...]

By |2016-12-20T11:20:17+00:00December 20, 2016|Nucleic Acid|Comments Off on Molecular Biology of Nucleic Acids | Microbiology
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Proteins (Exam Questions and Answers) | Microbiology

Everything you need to know about proteins. Some of the most frequently asked questions are as follows:- Q.1. What are proteins? Ans: A protein is a macromolecule composed of one or more polypeptide chains, each with a characteristic sequence of amino acids linked by peptide bonds. The cells of organisms contain thousands of different proteins each with a different function [...]

By |2016-12-20T11:20:15+00:00December 20, 2016|Proteins|Comments Off on Proteins (Exam Questions and Answers) | Microbiology
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