Most molecular markers fall into one of three basic categories of techniques that use either hybridization or are based on the polymerase chain reaction (PCR): Category # 1: Hybridization Based (non-PCR) Techniques: Category I includes restriction fragment length polymorphism (RFLP) analysis, where probes are hybridized to filters containing DNA which has been digested with restriction enzymes. The resultant fragments are [...]
In this article we will discuss about agarose gel electrophoresis. To know about quality and quantity of genomic DNA, agarose gel electrophoresis is performed on genomic and amplified DNA. Larger molecules migrate more slowly because of greater friction drag and they form their way through the pores of gel, less efficiently than smaller molecules. Range of separation in gel containing [...]
Justifications for the development and use of MAS in plant breeding fall into four broad areas that are relevant to almost all target crops. These are: a. Traits that are difficult to manage through conventional phenotypic selection because they are expensive or time consuming to measure, or have low penetrance or complex inheritance. b. Traits whose selection depends on specific [...]