In this article we will discuss about the mode of action of antisense RNA. Several possible mechanisms are involved during antisense inhibition of specific gene. Following are some of the most promising mode of actions have been suggested (Fig. 22.3). Action in the Nucleus Premise: One of the earliest methods in antisense inhibition is carried out by blocking transcription. This [...]
In this article we will discuss about the manipulation of fruit softening by antisense RNA. Plant cell wall is responsible for providing mechanical strength to plant organs. Tissue and cells due to their complex structure and biomolecules are involved. During fruit ripening several hydrolytic enzymes such as PG, PME and cellulase cleaves various polymers that constitute cell wall and consequently [...]
In this article we will demonstrate the expression vector that make antisense gene RNA in laboratory. Inhibition of gene expression in natural system by antisense RNA led to the development of strategies to artificially regulate genes using antisense RNA. In a single manipulation, antisense RNA complementary to target mRNA can be synthesized in laboratory and may be used to down [...]