In this article we will discuss about the antisense RNA in post harvesting improvement of plants. Engineering of Fruit Ripening Process: Efficacy of antisense RNA technology in post harvesting has been implicated well in extending shelf life of fruits, vegetables and vase life of flowers. Infact the first genetically modified products commercialized in markets were calgenes flavr savr tomatoes. Antisense [...]
Natural occurrence of antisense RNA and its role in gene regulation have been realised long ago. Gene expression in both prokaryotes and eukaryotes are controlled by the product of regulatory gene, which are supposed to be proteins only. Recently, naturally occurring regulatory gene that directs the synthesis of RNA which can control gene expression. This newly discovered RNA repressor can [...]
In this article we will discuss about the mode of action of antisense RNA. Several possible mechanisms are involved during antisense inhibition of specific gene. Following are some of the most promising mode of actions have been suggested (Fig. 22.3). Action in the Nucleus Premise: One of the earliest methods in antisense inhibition is carried out by blocking transcription. This [...]