Read this article to learn about the technical considerations required in the use of RNA interference. In several respects the approaches for silencing gene expression using RNAi methods are similar to those used for antisense DNA-mediated suppression of gene expression. In principle, any cloned gene can be targeted by designing RNA oligonucleotides or RNA-expressing viral vectors with sequences complementary to [...]
This article throws light upon the two useful applications of RNA Interference. The two useful applications are: (1) Medicine and (2) Biotechnology. Application # 1. Medicine: It may be possible to exploit RNA interference in therapy. Although it is difficult to introduce long dsRNA strands into mammalian cells due to the interferon response, the use of short interfering RNA mimics [...]
This article provides a close view on the RNA interference. Introduction: Cells use dicer to trim double stranded RNA to form small interfering RNA or microRNA. An exogenous dsRNA or endogenous pre-miRNA can be processed by dicer and incorporated into the RNA-induced silencing complex (RISC), which targets single-stranded messenger RNA molecules and triggers translational repression; incorporation into the RNA-induced transcriptional [...]