The below mentioned article provides the mechanism of protoplast fusion with six explanations. The mechanism of protoplast fusion is not fully known. Several explanations have been put forward to understand the mechanism of protoplast fusion. Some important explanations are given below: 1. When the protoplasts are brought into close proximity, this is followed by an induction phase whereby changes induced [...]
The following points highlight the two methods of protoplast fusion. They are: (1) Spontaneous Fusion and (2) Induced Fusion. Method # 1. Spontaneous Fusion: Protoplasts during isolation often fuse spontaneously and this phenomenon is called spontaneous fusion. Simply physical contact is sufficient to bring about the spontaneous fusion among the similar parental protoplasts. During the enzyme treatment for the isolation [...]
In the below mentioned article, we will discuss about the protocol for isolation and culture of protoplast. Protoplasts can be prepared from a variety of tissue but among them leaf mesophyll tissue from a wide range of plants has been proved to be the most ideal source of plant material for protoplast isolation (Fig 12.5). Leaves of Nicotiana tabacum is [...]