In this article we will discuss about the top two methods used for estimation of sugar in plants. The methods are: 1. Titrimetric Method 2. Colorimetric Method Using Anthrone Reagent. Method # 1. Titrimetric Method: Principle: All the sugars with free reducing group (aldehyde or ketone) undergo enolisation in presence of alkali. As a result they behave as strong reducing [...]
In this article we will discuss about the tests for estimation of free fatty acid. Principle: The free fatty acid in an oil is estimated by titrating it against KOH in the presence of phenolphthalein indicator. The acid number is defined as the mg KOH required to neutralize the free fatty acids present in 1 g of sample. However, the [...]
In this article we will discuss about the Kjeldahl method for the estimation of nitrogen in plants. Principle: Digestion of nitrogenous compounds by concentrated sulphuric acid in presence of catalysts (reduced iron powder, and a mixture of CuSO4 and K2SO4) produces ammonium compound. Further, such ammonium compound when distilled by steam in presence of strong alkali (40% NaOH soln.) produces [...]